Coding

Part:BBa_K3760003:Design

Designed by: Ha Buer   Group: iGEM21_LZU-CHINA   (2021-10-01)


T7 promoter + hACE2 target RNA coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 525
    Illegal BamHI site found at 1090
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2442
    Illegal SapI site found at 28
    Illegal SapI site found at 315


Design Notes

none


Source

we ues PCR to construct this part. hACE2 gene is from plasmid pLENTI_hACE2_PURO .

References